Journal: Cell reports

Article Title: β-Amyloid Clustering around ASC Fibrils Boosts Its Toxicity in Microglia

doi: 10.1016/j.celrep.2020.02.025

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Thereafter, cells were blocked using 5% normal goat serum (Vector Laboratories) in PTX for 20 min and primary antibodies were added for another 30 min. To check for ASC speck formation, the rabbit anti-ASC (1:250; clone AL177, AdipoGen) or mouse-specific rabbit anti-ASC/TMS1 (1:250, D2W8U, Cell Signaling Technology®) and rat anti-CD11b (1:250; Serotec by Bio-Rad) were used.

Techniques: Control, Recombinant, Magnetic Beads, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Quantitation Assay, Gel Extraction, Purification, Isolation, Software, Transmission Assay, Microscopy, Spectrophotometry, Fluorescence, Imaging

Journal: Molecular cell

Article Title: An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

doi: 10.1016/j.molcel.2023.05.018

Figure Lengend Snippet:

Article Snippet: Anti-ASC/TMS1 rabbit monoclonal antibody (E1E3I) , Cell Signaling Technology , cat# 13833; RRID: AB_2798325.

Techniques: Recombinant, Magnetic Beads, Transfection, Sequencing, Modification, Mass Spectrometry, Produced, Protease Inhibitor, Selection, Purification, Gel Extraction, SYBR Green Assay, Reporter Assay, Protein Quantitation, CRISPR, Amplification, Molecular Cloning, Plasmid Preparation, Software

Journal: PLoS Pathogens

Article Title: Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-β Responses

doi: 10.1371/journal.ppat.1005019

Figure Lengend Snippet: List of antibodies used in this study.

Article Snippet: Mouse monoclonal antibody against ASC was from MBL International, Woburn, MA.

Techniques:

Journal: Molecular cell

Article Title: An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

doi: 10.1016/j.molcel.2023.05.018

Figure Lengend Snippet:

Article Snippet: Anti- ASC/TMS1 mouse monoclonal antibody , Proteintech , cat# 67494-1-Ig; RRID: AB_2882718.

Techniques: Recombinant, Magnetic Beads, Transfection, Sequencing, Modification, Mass Spectrometry, Produced, Protease Inhibitor, Selection, Purification, Gel Extraction, SYBR Green Assay, Reporter Assay, Protein Quantitation, CRISPR, Amplification, Molecular Cloning, Plasmid Preparation, Software